abciximab  (Eli Lilly)

Journal: Blood Vessels, Thrombosis & Hemostasis

Article Title: Endo-chip laser-induced thrombus formation: a vessel-on-chip model for in vitro testing of antithrombotic agents

doi: 10.1016/j.bvth.2025.100096

Figure Lengend Snippet: Inhibition of thrombus formation by inhibitory antiplatelet antibody 7E3 (abciximab) but not by low dose aspirin in the Endo-chip laser-injury model. (A) Blood was incubated with vehicle, aspirin (9 μg/mL), or abciximab (20 μg/mL) for 1 hour at RT. Blood was then labeled with anti-CD41 antibody and antifibrin antibody, recalcified with CaCl 2 (10 mM), and then immediately perfused through the Endo-chip at 100 s –1 , followed by laser injury to the endothelial layer. Representative images of platelet (CD41, green) and fibrin (magenta) accumulation at the injury site at 10 minutes. (B) Kinetics of platelet (upper panel) and fibrin (lower panel) surface area coverage over time. (C) Platelet (upper panel) and fibrin (lower panel) AUC after treatment of blood with vehicle, aspirin, or abciximab. Mean ± SD, n = 3 to 5, 1-way analysis of variance with Dunnett’s multiple comparisons test. AUC, area under the curve.

Article Snippet: Abciximab (anti-αIIbβ3 inhibitory antibody, clone C7E3) was purchased from MedChemExpress LLC (Monmouth Junction, NJ).

Techniques: Inhibition, Incubation, Labeling

Journal: iScience

Article Title: Longitudinal analysis of genomic mutations in SARS-CoV-2 isolates from persistent COVID-19 patient

doi: 10.1016/j.isci.2024.109597

Figure Lengend Snippet: Sensitivity of isolated viruses to antiviral drugs and antibodies (A) Human induced pluripotent stem (iPS) cell-derived lung organoids were infected with a 0.1 multiplicity of infection (MOI) of isolated viruses and cultured for 4 days. Viral RNA copy number in the cell culture supernatant as measured by qPCR. Statistical significance between day 0 virus and other isolated viruses was determined by one-way analysis of variance (ANOVA) followed by Dunnett post hoc tests (∗ p < 0.05, ∗∗ p < 0.01). Data are shown as mean ± SD ( n = 3). (B) Human iPS cell-derived lung organoids were infected with 0.1 MOI of isolated viruses in the presence MOI or absence of remdesivir (RDV) or sotrovimab and cultured for 4 days. Viral RNA copy number in the cell culture supernatant as measured by qPCR. The EC50 of remdesivir or sotrovimab was determined. (C) Heatmap of the spike genome variations compared with the Wuhan-Hu-1 reference sequence. Genome mutations detected in all viruses are not shown. (D) SARS-CoV-2 variants with D574N or S1003I are summarized. The variant proportion with these mutations in each lineage. See also Figures S1–S3 .

Article Snippet: Sotrovimab , MedChemExpress , Cat# HY-P99340.

Techniques: Isolation, Derivative Assay, Infection, Cell Culture, Virus, Sequencing, Variant Assay

Journal: iScience

Article Title: Longitudinal analysis of genomic mutations in SARS-CoV-2 isolates from persistent COVID-19 patient

doi: 10.1016/j.isci.2024.109597

Figure Lengend Snippet:

Article Snippet: Sotrovimab , MedChemExpress , Cat# HY-P99340.

Techniques: Virus, Recombinant, Membrane, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Software, Cell Culture, Real-time Polymerase Chain Reaction, Flow Cytometry